Guo, Binhui; Kai, Guoyin; Gong, Yi-fu; Jin, Hongbin; Wang, Yechun; Miao, Zhiqi; Sun, Xiao-fen; Tang, Ke-xuan

MOLECULAR CLONING AND HETEROLOGOUS EXPRESSION OF A 10-DEACETYLBACCATIN III-10-O-ACETYL TRANSFERASE CDNA FROM TAXUS X MEDIA

A full-length cDNA encoding 10-deacetylbaccatin III-10-O-acetyl transferase (designated as TmDBAT), which catalyzes the acetylation of the C-10 hydroxyl group of the advanced metabolite 10-deacetylbaccatin III (10-DAB) to yield baccatin III, the immediate diterpenoid precursor of Taxol, was isolated from Taxus x media. Heterologous expression of TmDBAT in E. coli demonstrated that TmDBAT was a functional gene. Tissue expression pattern analysis revealed that TmDBAT expressed strongly in leaves, weak in stems and no expression could be detected in fruits, implying that TmDBAT was tissue-specific. Expression profiling analysis of TmDBAT under different elicitor treatments including silver nitrate, ammonium ceric sulphate and methyl jasmonate indicated that TmDBAT was an elicitor-responsive gene. Southern blot analysis suggested that TmDBAT belonged to a small multigene family.

Pubmed ID: 17094009

Keywords: Larix chinensisELarix chinensisSLarix chinensisTLarix chinensis, Larix chinensisCLarix chinensisDLarix chinensisNLarix chinensisALarix chinensis, Larix chinensisTLarix chinensisALarix chinensisXLarix chinensisULarix chinensisSLarix chinensis Larix chinensisXLarix chinensis Larix chinensisMLarix chinensisELarix chinensisDLarix chinensisILarix chinensisALarix chinensis, Larix chinensisTLarix chinensisALarix chinensisXLarix chinensisULarix chinensisSLarix chinensis, Larix chinensisDLarix chinensisNLarix chinensisALarix chinensis

Molecular Biology Reports, 2007; Vol 34(2) : 89 - 95