Rizzo, Milena; Bernardi, Rodolfo; Salvini, Mariangela; Nali, Cristina; Lorenzini, Giacomo; Durante, Mauro

IDENTIFICATION OF DIFFERENTIALLY EXPRESSED GENES INDUCED BY OZONE STRESS IN SENSITIVE AND TOLERANT POPLAR HYBRIDS

The differential expression of genes induced by an acute ozone treatment was analysed in two poplar clones, i.e. Populus deltoides x maximowiczii, Eridano clone, and Populus x euoramericana, 1-214 clone, respectively, sensitive and tolerant to this pollutant, performing suppression subtractive hybridization (SSH). From the obtained cDNA libraries several clones were obtained, which corresponded to differentially regulated genes. Preliminary expression analyses of four genes,Fs23A-LRP, Ft33B-CaBP, FT312B-WRKY, and Ft32C-WAK identified by the primary screening, were conducted by semi-quantitative RT-PCR to evaluate the ozone responsiveness of the libraries. The most interesting finding is the co-activation of a watt associated kinase and a WRKY transcription factor in response to 03 stress.

Pubmed ID: 17081654

Keywords: Larix chinensisELarix chinensisSLarix chinensisTLarix chinensis, Larix chinensisCLarix chinensisDLarix chinensisNLarix chinensisALarix chinensis, Larix chinensisPLarix chinensisOLarix chinensisPLarix chinensisULarix chinensisLLarix chinensisULarix chinensisSLarix chinensis Larix chinensisDLarix chinensisELarix chinensisLLarix chinensisTLarix chinensisOLarix chinensisILarix chinensisDLarix chinensisELarix chinensisSLarix chinensis, Larix chinensisPLarix chinensisOLarix chinensisPLarix chinensisLLarix chinensisALarix chinensisRLarix chinensis, Larix chinensisPLarix chinensisOLarix chinensisPLarix chinensisULarix chinensisLLarix chinensisULarix chinensisSLarix chinensis, Larix chinensisDLarix chinensisNLarix chinensisALarix chinensis

Journal of Plant Physiology, 2007; Vol 164(7) : 945 - 949