| Preliminary list of host genes significant in large fusiform rust galls vs. small fusiform rust galls | |||||||
| Arrays of 323 genes (redundant #) were probed with RNA from xylem or phloem isolated from either small or large galls | |||||||
| No multiple testing corrections performed - therefore, at a=0.1 and 323 genes, we expect 32 genes to be significant by chance. | |||||||
| Although 11 of the putatively significant host genes were also significant in the Fusarium experiment, 9 of them were regulated in opposite directions | |||||||
| significance test: | |||||||
| Type 2 = large (Lg) vs small (Sm) galls | |||||||
| Type 3 = large phloem (LG P) vs small phloem (Sm P) | |||||||
| Type 4 = large xylem (Lg X) vs small xylem (Sm X) | |||||||
| Significance test | |||||||
| clone | treatment | Assigned 'functions' (E value <10-5) |
Type 2 Lg vs Sm |
Type 3 Lg P vs Sm P |
Type 4 Lg X vs Sm X |
Direction of Regulation | l significantly up-regulated by Fusarium |
| pi129 | salicylate | CCoAOMT | 1 | 1 | down-reg in Lg P | ||
| pi231 | F.R. suscept. | p3H6 | 1 | 1 | down-reg Lg X | ||
| pi213 | P.C. resist. | PAL | 1 | 1 | down-reg in Lg X | ||
| pi240 | P.C. resist. | phenylcoumaran benzylic ether reductase | 1 | 1 | 1 | down-reg in Lg P and Lg X | |
| catechol-OMT | 1 | 1 | down-reg in Lg X | ||||
| pi287 | P.C. suscept. | ABC transporter-like protein | 1 | 1 | up-reg Lg P | l | |
| pi140 | salicylate | putative ARF6 | 1 | down-reg in Lg | |||
| H12 | healthy | putative MADS box protein | 1 | 1 | down-reg in Lg P | ||
| MAPK2 | regulatory | 1 | down-reg in Lg | ||||
| Myb 126 | regulatory | 1 | up-reg in Lg P | ||||
| pi318 | F.R. suscept. | RNA binding domain protein | 1 | 1 | down-reg lg P | ||
| pi314 | F.R. suscept. | RNA binding protein | 1 | down-reg in Lg | |||
| H37 | healthy | callose synthase | 1 | 1 | down-reg in Lg P | ||
| pi305 | P.C. suscept. | Catalase isozyme 2 | 1 | down-reg in Lg | |||
| pi203 | P.C. resist. | putative gamma-thionin precursor | 1 | 1 | down-reg Lg X | ||
| pi185 | P.C. resist. | putative LRR protein | 1 | down-reg in Lg | |||
| pidd1 | wound | class I chitinase | 1 | 1 | 1 | down-reg Lg P and Lg X | l |
| pi279, pi303, pi304 | P.C. suscept. | class III peroxidase | 1 | down-reg Lg X | l | ||
| pi73 | salicylate | class IV chitinase | 1 | down-reg in lg | |||
| pi326 | F.R. suscept. | DNA-damage-repair/toleration protein | 1 | 1 | 1 | up-reg Lg P and Lg X | |
| H7 | healthy | heat shock protein | 1 | 1 | down-reg in Lg X | ||
| pi253 | P.C. suscept. | embryo abundance protein EMB20 | 1 | 1 | down-reg Lg P | ||
| pi164 | P.C. resist. | embryonic abundant protein EMB32 | 1 | 1 | 1 | down-reg in Lg P and Lg X | |
| H25 | healthy | Metallothionein-like protein EMB30 | 1 | 1 | 1 | down-reg in Lg P and Lg X | |
| H13 | healthy | endoxyloglucan transferase | 1 | 1 | down-reg in Lg P | ||
| H17 | healthy | glyceraldehde 3 -P dehydrogenase | 1 | down-reg in Lg | |||
| G40 | galled | glyceraldehde 3 -P dehydrogenase | 1 | 1 | down-reg in Lg P | ||
| H23 | healthy | MtN3-like | 1 | 1 | 1 | down-reg in Lg P and Lg X | |
| pi193 | P.C. resist. | NADH dehydrogenase | 1 | 1 | down-reg in Lg P | ||
| pic54 | chitosan | NADH dehydrogenase | 1 | 1 | down-reg Lg P | ||
| H24 | healthy | NADH dehydrogenase | 1 | 1 | down-reg in Lg P | ||
| H19 | healthy | NAM | 1 | 1 | down-reg in Lg P | ||
| pi317 | F.R. suscept. | Ribose 5-P isomerase | 1 | 1 | down-reg Lg X | ||
| pi284 | P.C. suscept. | ribosomal protein L1 | 1 | 1 | up-reg Lg P | l | |
| G37 | galled | ribosomal protein L17 | 1 | 1 | up-reg in Lg P | ||
| J6 | F.R. suscept. | tubulin alpha 1 chain | 1 | up-reg in lg P | |||
| G12 | healthy | No similaritity | 1 | up-reg in Lg | |||
| G36 | healthy | No similaritity | 1 | up-reg in Lg P | |||
| G39 | healthy | No similaritity | 1 | down-reg in Lg | |||
| pi204 | PC R | No similaritity | 1 | down-reg in Lg | |||
| pi228a | F.R. suscept. | No similaritity | 1 | down-reg in Lg | l | ||
| pi311a | F.R. suscept. | No similaritity | 1 | up-reg in Lg | |||
| pi97 | salicylate | No similaritity | 1 | down-reg Lg | |||
| H1 | healthy | No similaritity | 1 | down-reg in Lg P | |||
| H41 | healthy | No similaritity | 1 | up-reg in L P | |||
| pi233 | F.R. suscept. | No similaritity | 1 | down-reg Lg X | |||
| G50 | galled | No similaritity | 1 | 1 | up-reg in Lg P | ||
| G73 | galled | No similaritity | 1 | 1 | down-reg in Lg P | ||
| G75 | galled | No similaritity | 1 | 1 | down-reg in Lg P | ||
| pi205 | P.C. resist. | No similaritity | 1 | 1 | down-reg in Lg P | ||
| pi226 | F.R. suscept. | No similaritity | 1 | 1 | down-reg Lg P | ||
| pi252 | P.C. suscept. | No similaritity | 1 | 1 | down-reg Lg P | ||
| pi260 | P.C. suscept. | No similaritity | 1 | 1 | up-reg Lg P | ||
| pidd13 | wound | No similaritity | 1 | 1 | down-reg Lg P | ||
| H35 | healthy | No similaritity | 1 | 1 | down-reg in Lg P | ||
| G41 | galled | No similaritity | 1 | 1 | 1 | up in Lg P and Lg X | |
| G45 | galled | No similaritity | 1 | 1 | 1 | up in Lg P and Lg X | |
| G56 | galled | No similaritity | 1 | 1 | 1 | up in Lg P and Lg X | |
| G65 | galled | No similaritity | 1 | 1 | 1 | up in Lg P and Lg X | |
| G72 | galled | No similaritity | 1 | 1 | 1 | down-reg in Lg P and Lg X | |
| H34 | healthy | No similaritity | 1 | 1 | 1 | down-reg in Lg P and Lg X | |
| pi235 | F.R. suscept. | No similaritity | 1 | 1 | 1 | up in Lg P and Lg X | |
| pi245 | F.R. suscept. | No similaritity | 1 | 1 | 1 | no signal in large galls…. | |
| pi261 | P.C. suscept. | No similaritity | 1 | 1 | 1 | up in Lg P and Lg X | |
| pi266 | P.C. suscept. | No similaritity | 1 | 1 | 1 | down-reg Lg P and Lg X | |
| pi323 | F.R. suscept. | No similaritity | 1 | 1 | 1 | up Lg P and Lg X | |
| H6 | healthy | No similaritity | 1 | 1 | down-reg in Lg X | ||
| pi173 | P.C. resist. | No similaritity | 1 | 1 | down-reg in Lg X | ||
| pi306 | P.C. suscept. | No similaritity | 1 | 1 | down-reg Lg X | ||
| pi280 | P.C. suscept. | No similaritity | 1 | 1 | down-reg Lg X | l | |
| G51 | galled | pine EST | 1 | 1 | up-reg in Lg P | ||
| H10 | healthy | pine EST | 1 | down-reg in Lg | |||
| H14 | healthy | pine EST | 1 | 1 | down-reg in Lg P | ||
| H21 | healthy | pine EST | 1 | 1 | down-reg in Lg P | ||
| H32 | healthy | pine EST | 1 | down-reg in Lg X | |||
| H38 | healthy | pine EST | 1 | 1 | 1 | down-reg in Lg P and Lg X | |
| J4 | F.R. suscept. | pine EST | 1 | 1 | up-reg in Lg P | ||
| J7 | F.R. suscept. | pine EST | 1 | 1 | up-reg in Lg P | ||
| pi121 | salicylate | pine EST | 1 | 1 | down-reg in Lg P | ||
| pi150, pi125 | salicylate | pine EST | 1 | 1 | down-reg in Lg P | ||
| pi139 | salicylate | pine EST | 1 | 1 | down-reg in Lg P | l | |
| pi166, pi167 | P.C. resist. | pine EST | 1 | 1 | down-reg in Lg P | ||
| pi174 | P.C. resist. | pine EST | 1 | 1 | down-reg in Lg X | ||
| pi188 | P.C. resist. | pine EST | 1 | down-reg in Lg P | |||
| pi192 | P.C. resist. | pine EST | 1 | 1 | down-reg in Lg P | ||
| pi194, pi196 | P.C. resist. | pine EST | 1 | 1 | down-reg in Lg X | ||
| pi197 | P.C. resist. | pine EST | 1 | 1 | down-reg in Lg X | ||
| pi210, pi211, pi212 | P.C. resist. | pine EST | 1 | 1 | down-reg Lg P | ||
| pi237 | F.R. suscept. | pine EST | 1 | 1 | down-reg Lg X | ||
| pi264 | P.C. suscept. | pine EST | 1 | 1 | down-reg Lg X | ||
| pi277 | P.C. suscept. | pine EST | 1 | down-reg in Lg | l | ||
| pi310 | SA | pine EST | 1 | 1 | 1 | down-reg in Lg P and Lg X | |
| pi76 | salicylate | pine EST | 1 | down-reg in Lg | l | ||
| pi90 | salicylate | pine EST | 1 | 1 | down-reg Lg P | ||
| pic56 | chitosan | pine EST | 1 | down-reg Lg | |||
| pidd6 | wound | pine EST | 1 | down-reg Lg P | l | ||
| pi130 | salicylate | plant EST | 1 | down-reg in Lg | l | ||
| G11 | galled | plant EST | 1 | up-reg in Lg P | |||
| G54 | galled | plant EST | 1 | 1 | down-reg in Lg P | ||
| G55 | galled | plant EST | 1 | 1 | up in Lg P | ||
| H11 | healthy | plant EST | 1 | 1 | 1 | down-reg in Lg P and Lg X | |
| H39 | healthy | plant EST | 1 | 1 | 1 | down-reg in Lg P and Lg X | |
| H5 | healthy | plant EST | 1 | 1 | down-reg in Lg X | ||
| pidd18 | wound | unclassified | 1 | down-reg Lg | |||
| G53 | galled | unclassified | 1 | 1 | up-reg in Lg P | ||
| H33 | healthy | unclassified | 1 | 1 | down-reg in Lg P | ||
| H22 | healthy | unclassified | 1 | 1 | down-reg in Lg X | ||
| pi291 | P.C. suscept. | unclassified | 1 | 1 | down-reg Lg X | ||
| pi319 | F.R. suscept. | unclassified | 1 | 1 | down-reg Lg X | ||
| pi77 | salicylate | unclassified | 1 | 1 | down-reg Lg X | ||
| J5 | F.R. suscept. | unclasssified | 1 | 1 | down-reg in Lg X | ||
| pic23 | chitosan | unclasssified | 1 | 1 | down-reg Lg P | ||