Table 2. Summary of Fusarium-regulated cDNAs at 8 days post-challenge.
|
Clone |
Treatmenta |
Annotationb |
E-valuec |
Speciesd |
Fold inductione |
Bonferonni adjusted p values |
|
pi273 |
P.C. suscept. |
Unknown, AAM62667.1 (X) |
2.00E-08 |
Arabidopsis thaliana |
26.4 |
0.000 |
|
Pechi270 |
P.C. suscept. |
Class IV chitinase, AAL58880.1 (X) |
4.00E-45 |
Pinus elliottii |
12.0 |
0.000 |
|
pi279, pi303, pi272, pi304 |
P.C. suscept. |
Class III peroxidase, AAG02215.1 (X) |
1.00E-25 |
Pinus sylvestris |
9.6 |
0.000 |
|
pi277, pi278, pi275 |
P.C. suscept. |
cDNA clone, AI919956 (E) |
1.00E-35 |
Pinus taeda |
2.6 |
0.000 |
|
pi293 |
P.C. suscept. |
no similarity |
>10-5 |
- |
2.8 |
0.001 |
|
pi276, pi292 |
P.C. suscept. |
Tar1p, NP_690845.1 (X) |
6.00E-18, |
Saccharomyces cerevisiae |
2.1 |
0.002 |
|
pi64 |
P.C. resist. |
PR10-related protein, AF197342.1 (X) |
2.00E-27 |
Picea glauca |
10.6 |
0.006 |
|
pi284 |
P.C. suscept. |
Ribosomal protein L1, T51935 (X) |
1.00E-38 |
Spinacia oleracea |
24.8 |
0.009 |
|
pi117 |
salicylate |
Antimicrobial peptide 2, AL05053.1 (bestEST) |
1.00E-102, 9.00E-59 |
Pinus sylvestris |
2.2 |
0.014 |
|
pi274 |
P.C. suscept. |
no similarity |
>10-5 |
- |
1.6 |
0.024 |
|
pi263 |
P.C. suscept. |
Xylem cDNA, BQ700839.1 (E) |
6.00E-31 |
Pinus taeda |
2.3 |
0.026 |
|
Pechi1 |
wounded |
Putative class I chitinase, AAL58875.1 (X) |
2.00E-32 |
Pinus elliottii |
11.8 |
0.027 |
|
pi280 |
P.C. suscept. |
no similarity |
>10-5 |
- |
1.7 |
0.032 |
|
pi76, pi128 |
salicylate |
cDNA clone, AA739574.1 (E) |
1.00E-147 |
Pinus taeda |
2.2 |
0.032 |
|
pi287 |
P.C. suscept. |
ABC transporter-like protein, NP_191829.1 (bestEST) |
5.00E-47 |
Arabidopsis thaliana |
2.4 |
0.037 |
|
pi297 |
P.C. suscept. |
Pollen cone cDNA, AW754815 (E) |
2.00E-35 |
Pinus taeda |
3.7 |
0.037 |
|
pi250 |
P.C. suscept. |
no similarity |
>10-5 |
- |
4.2 |
0.047 |
|
pi289 |
P.C. suscept. |
Hydrophobin 3, AAO16869.1 (bestEST) |
1.00E-102, 3.00E-15 |
Gibberella moniliformis |
3.0 |
0.055 |
|
pi156-2 |
salicylate |
Xylem cDNA, BQ107663.1 (E) |
2.00E-69 |
Pinus taeda |
2.4 |
0.066 |
|
pi228a |
F.R. suscept. |
no similarity |
>10-5 |
- |
2.4 |
0.066 |
|
pi302 |
P.C. suscept. |
Xylem cDNA, BF609752.1 (E) |
1.00E-140 |
Pinus taeda |
2.3 |
0.076 |
|
pi301 |
P.C. suscept. |
Expressed protein, NP_564667.1, (X) |
1.00E-20 |
Arabidopsis thaliana |
2.6 |
0.084 |
|
pi307a |
P.C. suscept. |
Boiling stable protein A, CAC34953.1 (X) |
3.00E-09 |
Populus |
2.2 |
0.085 |
|
pi139 |
salicylate |
Xylem cDNA, BI202925.1 (E) |
1.00E-10 |
Pinus taeda |
2.3 |
0.090 |
|
pi130 |
salicylate |
cDNA library, BI416804.1 (E) |
3.00E-62 |
Heterobasidion annosum or Pinus sylvestris |
2.3 |
0.093 |
|
pidd6-2 |
wound |
Xylem cDNA, BQ197534.1 (E) |
3.00E-71 |
Pinus taeda |
2.5 |
0.094 |
|
pi152-5 |
salicylate |
Actin-depolymerizing factor, Q8SAG3 (bestEST) |
1.00E-101, 9.00E-52 |
Pinus taeda |
2.5 |
0.096 |
|
pi281 |
P.C. suscept. |
no similarity |
>10-5 |
- |
1.7 |
0.098 |
|
pi307b |
P.C. suscept. |
Photosystem II subunit, P16059 (X) |
3.00E-29 |
Pisum sativum |
2.3 |
0.099 |
a pitch canker (P.C.) resistant and susceptible plants were challenged with F. circinatum, and fusiform rust (F.R.) susceptible plants were challenged with C. q. fusiforme.
b Annotation based on: X=BLASTX, E=BLASTn against dbEST, bestEST=annotated using EST sequence with the best sequence match as a blastx query.
c E-values >10-5 were considered not similar
d Species of origin for the top BLAST hit
e For genes represented by multiple cDNAs, the expression ratios (diseased/control) are given as averages.